LINC00470 represses cell autophagy and cisplatin sensitivity
of glioma via suppressing PTEN expression

Biyin Chen; Wenwu Wang; Fangfeng Lin; Shuping Shi; Shunjie Ou; Yunqiu Yu

doi:10.5114/fn.2023.125327

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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abstract:

Original paper

LINC00470 represses cell autophagy and cisplatin sensitivity of glioma via suppressing PTEN expression

Biyin Chen

¹

,

Wenwu Wang

¹

,

Fangfeng Lin

¹

,

Shuping Shi

¹

,

Shunjie Ou

¹

,

Yunqiu Yu

¹

The Third Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, China

Folia Neuropathol 2023; 61 (1): 88-96

DOI: https://doi.org/10.5114/fn.2023.125327

Online publish date: 2023/02/23

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Introduction:
Glioma is one of primary brain tumours which has the worst clinical prognoses of patients. As an alternative chemotherapeutic drug for malignant glioma, the therapeutic effect of cisplatin (CDDP) is devastatingly affected due to resistance in patients. In this study, we investigated the effect of LINC00470/PTEN on the CDDP sensitivity of glioma cells.

Material and methods:
Differentially expressed lncRNAs and the downstream regulators in glioma tissue were obtained via bioinformatics analysis. LINC00470 and PTEN mRNA expression levels were detected using qRT-PCR. IC50 values of glioma cells were examined using Cell Counting Kit-8 (CCK-8). Cell apoptosis was revealed by flow cytometry. The expression level of autophagy-related protein was detected by western blot. Intracellular autophagosome formation was detected by immunofluorescence staining, and the methylation level of PTEN promoter was detected via methylation-specific PCR (MSP).

Results:
Through the above steps, we found that LINC00470 was highly expressed in glioma cells, and the survival rate of patients was reduced in the presence of high expression of LINC00470. Silenced LINC00470 promoted LC3 II expression and autophagosome formation, and facilitated cell apoptosis to inhibit resistance to CDDP. While silenced PTEN could successfully reverse the previous effects on glioma cells.

Conclusions:
Based on the above, LINC00470 repressed cell autophagy by constraining PTEN, thereby enhancing CDDP resistance of glioma cells.

keywords:

LINC00470 represses cell autophagy and cisplatin sensitivity of glioma via suppressing PTEN expression

Biyin Chen 1 , Wenwu Wang 1 , Fangfeng Lin 1 , Shuping Shi 1 , Shunjie Ou 1 , Yunqiu Yu 1

LINC00470, PTEN, autophagy, glioma, cisplatin resistance

Biyin Chen

¹

,

Wenwu Wang

¹

,

Fangfeng Lin

¹

,

Shuping Shi

¹

,

Shunjie Ou

¹

,

Yunqiu Yu

¹