Lipopolysaccharide-based endotoxemia produce toxicity in peripheral organs and microglia migration in a dose-dependent manner in rat substantia nigra

Paola Heman Bozadas; Rasajna Nadella; Aurora Sánchez-García; Daniel Rembao Bojórquez; María Rovirosa Hernández; Daniel Hernandez Baltazar

doi:10.5114/fn.2019.88454

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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3/2019
vol. 57

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abstract:

Original paper

Lipopolysaccharide-based endotoxemia produce toxicity in peripheral organs and microglia migration in a dose-dependent manner in rat substantia nigra

Paola Heman Bozadas

¹

,

Rasajna Nadella

²

,

Aurora Sánchez-García

³

,

Daniel Rembao Bojórquez

³

,

María Rovirosa Hernández

¹

,

Daniel Hernandez Baltazar

⁴

Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, Mexico
IIIT Srikakulam, Rajiv Gandhi University of Knowledge Technologies (RGUKT), international collaboration ID: 1840, India
Instituto Nacional de Neurología y Neurocirugía, Ciudad de Mexico, Mexico
CONACYT-Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, Mexico

Folia Neuropathol 2019; 57 (3): 258-266

DOI: https://doi.org/10.5114/fn.2019.88454

Online publish date: 2019/09/30

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AMA

Heman Bozadas P, Nadella R, Sánchez-García A, Rembao Bojórquez D, Rovirosa Hernández M, Hernandez Baltazar D. Lipopolysaccharide-based endotoxemia produce toxicity in peripheral organs and microglia migration in a dose-dependent manner in rat substantia nigra. Folia Neuropathologica. 2019;57(3):258-266. doi:10.5114/fn.2019.88454.

APA

Heman Bozadas, P., Nadella, R., Sánchez-García, A., Rembao Bojórquez, D., Rovirosa Hernández, M.,  & Hernandez Baltazar, D. (2019). Lipopolysaccharide-based endotoxemia produce toxicity in peripheral organs and microglia migration in a dose-dependent manner in rat substantia nigra. Folia Neuropathologica, 57(3), 258-266. https://doi.org/10.5114/fn.2019.88454

Chicago

Heman Bozadas, Paola, Rasajna Nadella, Aurora Sánchez-García, Daniel Rembao Bojórquez, María Rovirosa Hernández,  and Daniel Hernandez Baltazar. 2019. "Lipopolysaccharide-based endotoxemia produce toxicity in peripheral organs and microglia migration in a dose-dependent manner in rat substantia nigra". Folia Neuropathologica 57 (3): 258-266. doi:10.5114/fn.2019.88454.

Harvard

Heman Bozadas, P., Nadella, R., Sánchez-García, A., Rembao Bojórquez, D., Rovirosa Hernández, M.,  and Hernandez Baltazar, D. (2019). Lipopolysaccharide-based endotoxemia produce toxicity in peripheral organs and microglia migration in a dose-dependent manner in rat substantia nigra. Folia Neuropathologica, 57(3), pp.258-266. https://doi.org/10.5114/fn.2019.88454

MLA

Heman Bozadas, Paola et al. "Lipopolysaccharide-based endotoxemia produce toxicity in peripheral organs and microglia migration in a dose-dependent manner in rat substantia nigra." Folia Neuropathologica, vol. 57, no. 3, 2019, pp. 258-266. doi:10.5114/fn.2019.88454.

Vancouver

Heman Bozadas P, Nadella R, Sánchez-García A, Rembao Bojórquez D, Rovirosa Hernández M, Hernandez Baltazar D. Lipopolysaccharide-based endotoxemia produce toxicity in peripheral organs and microglia migration in a dose-dependent manner in rat substantia nigra. Folia Neuropathologica. 2019;57(3):258-266. doi:10.5114/fn.2019.88454.

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The peripheral inflammatory stimulus could induce cell damage in peripheral organs and activate microglial cells in the brain. One such stimulus was given to adult male Wistar rats by injecting different concentrations of lipopolysaccharide (LPS; 50, 300, 500 g/kg and 5 mg/kg i.p.). To verify the systemic effect of the LPS administration, the serum content of C-reactive protein (CRP), the variation of body weight and cellular changes in the spleen, liver and kidney were determined. Motor impairment was evaluated by rotarod and open field tests. Microglia activation and dopaminergic degeneration was confirmed by immunolabelling for CD11b/c (microglia) and tyrosine hydroxylase (TH), respectively. The cell counting was performed in substantia nigra pars compacta (SNpc), microglial activation was explored in SNpc, substantia nigra pars reticulata (SNpr), substantia nigra pars compacta dorsal (SNcd) and the ventral tegmental area (VTA). For the statistical analysis, one-way ANOVA followed by Tukey post hoc test (p ≤ 0.05) was used. On day 7 post intraperitoneal administration of LPS, cellular atrophy was detected in the liver, kidney and spleen at 5 mg/kg, without significant changes in CRP levels. Body weight loss and motor impairment was present only on day 1 post LPS administration. The dosage of 500 g/kg and 5 mg/kg of LPS caused the loss of dopaminergic neurons (40%) in SNpc and microglia migration in a dose-dependent manner in SNcd, SNpc and SNpr. LPS-induced endotoxemia favours damage to the peripheral organs and microglial migration in a dose-dependent manner in rat substantia nigra.

keywords:

Lipopolysaccharide-based endotoxemia produce toxicity in peripheral organs and microglia migration in a dose-dependent manner in rat substantia nigra

Paola Heman Bozadas 1 , Rasajna Nadella 2 , Aurora Sánchez-García 3 , Daniel Rembao Bojórquez 3 , María Rovirosa Hernández 1 , Daniel Hernandez Baltazar 4

inflammation, microglia, dopamine, neurons, motor impairment

Paola Heman Bozadas

¹

,

Rasajna Nadella

²

,

Aurora Sánchez-García

³

,

Daniel Rembao Bojórquez

³

,

María Rovirosa Hernández

¹

,

Daniel Hernandez Baltazar

⁴