Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring

Lingxing Wang; Honghong Huang; Hongchao Cai; Yafang Chen

doi:10.5114/fn.2017.72392

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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4/2017
vol. 55

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abstract:

Original paper

Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring

Lingxing Wang

,

Honghong Huang

,

Hongchao Cai

,

Yafang Chen

Folia Neuropathol 2017; 55 (4): 317-324

DOI: https://doi.org/10.5114/fn.2017.72392

Online publish date: 2017/12/29

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Introduction: Maternal hypoxia induces an adverse uterine environment and may induce long-term effects in offspring. This study investigated whether maternal hypoxia increases hippocampal cell vulnerability and exacerbates neurological impairments in adult rat offspring following ischemia.

Material and methods: Pregnant Sprague-Dawley rats were randomly assigned to no maternal hypoxia or maternal hypoxia treatment groups. Adult male rat offspring were subjected to middle cerebral artery occlusion (MCAO). There were four groups: maternal + sham (MH + Sham), sham (Sham), maternal hypoxia + MCAO (MH + MCAO), and MCAO only (MCAO). Neurological deficits were evaluated. Hippocampal cell damage was observed by hematoxylin and eosin (HE) staining. Cell apoptosis in the hippocampus was detected by TdT-mediated dUTP-biotin nick-end labeling (TUNEL) staining. Caspase-3, cytochrome c, Bax, and bcl-2 expression in the hippocampus was detected by Western blot.

Results: More severe hippocampal cell damage was found in the MH + MCAO group than in the MCAO group. Additionally, neurological deficits, percentage of TUNEL positive cells, and expression of caspase-3, cytochrome c, and Bax in the hippocampus were significantly higher (p < 0.05), whereas bcl-2 expression was significantly lower (p < 0.05) in the MH + MCAO group compared to the MCAO group.

Conclusions: These findings suggest that maternal hypoxia may exacerbate hippocampal cell apoptosis in rat offspring after MCAO via alterations in the expression of cytochrome c, caspase-3, Bax, and bcl-2, which ultimately affects ischemic stroke prognosis. To our knowledge, this is the first study demonstrating that maternal hypoxia increases hippocampal cell susceptibility to ischemia in adult rat offspring.

keywords:

Maternal hypoxia increases hippocampal cell susceptibility to ischemia after middle cerebral artery occlusion in rat offspring

Lingxing Wang , Honghong Huang , Hongchao Cai , Yafang Chen

maternal hypoxia, offspring, cerebral ischemia, hippocampus, disease susceptibility

Lingxing Wang

,

Honghong Huang

,

Hongchao Cai

,

Yafang Chen