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ISSN: 0031-3939
Pediatria Polska - Polish Journal of Paediatrics
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2/2020
vol. 95
 
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abstract:
Original paper

Methylation status of leptin receptor gene promoter in obese children

Katarzyna Jakubek-Kipa
1
,
Natalia Potocka
2
,
Marzena Skrzypa
2
,
Łukasz Dembinski
3
,
Izabela Zawlik
2
,
Artur Mazur
1, 2

  1. II Clinic of Paediatrics, Endocrinology and Diabetology, Clinical Hospital no. 2 in Rzeszow, Rzeszow, Poland
  2. Centre for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of Rzeszow, Rzeszow, Poland
  3. Department of Paediatric Gastroenterology and Nutrition, Medical University of Warsaw, Warsaw, Poland
Pediatr Pol 2020; 95 (2): 86–91
DOI: https://doi.org/10.5114/polp.2020.97117
Online publish date: 2020/07/10
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Obesity in children is a growing problem in many countries around the world. Molecular processes have been identified that may lead to epigenetic patterns contributing to obesity, such as DNA methylation in promoters of specific genes. The leptin receptor gene (LEPR) has been associated with obesity, and the aim of our study was to evaluate the methylation status of the LEPR promoter in the white blood cells (WBC) of 50 children with obesity. Leptin receptor gene promoter methylation was analysed using Combined Bisulfite Restriction Analysis (COBRA). In all patients, the LEPR promoter was found to be unmethylated. The results may indicate that methylation of CpG islands in the LEPR promoter is not associated with obesity in children.
keywords:

obesity, children, methylation