MiR-125a-5p silencing inhibits cerebral ischemia-induced injury
through targeting IGFBP3

Bo Song; Jing Xu; Ping Zhong; Lei Fang

doi:10.5114/fn.2021.107109

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures

Editorial System

Submit your Manuscript

Editorial Policies

Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

2/2021
vol. 59

Send email

Copy url:

abstract:

Original paper

MiR-125a-5p silencing inhibits cerebral ischemia-induced injury through targeting IGFBP3

Bo Song

¹

,

Jing Xu

¹

,

Ping Zhong

¹

,

Lei Fang

¹

Department of Neurology, Qingdao Central Hospital, Qingdao, Shandong, China

Folia Neuropathol 2021; 59 (2): 121-130

DOI: https://doi.org/10.5114/fn.2021.107109

Online publish date: 2021/06/30

View full text Get citation

PlumX metrics:

Dysregulated microRNAs (miRNAs) are crucial regulators of cerebrovascular conditions, including ischemic stroke. Circulating miR-125a-5p is associated with ischemic stroke and may have clinical utility as an early diagnostic biomarker. This study conducted a series of experiments that were designed to elucidate the regulatory action of miR-125a-5p in ischemic brain injury and its underlying mechanisms. The results of this study found that the expression of miR-125a-5p was increased in BV2 microglial cells under oxygen-glucose deprivation/reoxygenation (OGD/R) setting, as well as in rat brain tissue after middle cerebral artery occlusion (MCAO). OGD/R triggered BV2 microglial cell apoptosis, whereas downregulation of miRNA-125a-5p suppressed the apoptosis rate in OGD/R-induced BV2 microglial cells. Subsequently, insulin-like growth factor binding protein 3 (IGFBP3) is a molecular target of miR-125a-5p, and IGFBP3 knockdown reversed the effects of miR-125a-5p inhibitor on BV2 microglial cells in vitro. These data supported the fact that miR-125a-5p silencing protects against cerebral ischemia-induced injury by targeting IGFBP3.

keywords:

MiR-125a-5p silencing inhibits cerebral ischemia-induced injury through targeting IGFBP3

Bo Song 1 , Jing Xu 1 , Ping Zhong 1 , Lei Fang 1

miR-125a-5p, IGFBP3, apoptosis, ischemic stroke

Bo Song

¹

,

Jing Xu

¹

,

Ping Zhong

¹

,

Lei Fang

¹