Myricitrin attenuates memory impairment in a rat model of sepsis-associated encephalopathy via the NLRP3/Bax/Bcl pathway

Junzuo Gong; Shu Luo; Shiqiao Zhao; Songlin Yin; Xiaofeng Li; Tianyi Mou

doi:10.5114/fn.2019.89856

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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4/2019
vol. 57

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abstract:

Original paper

Myricitrin attenuates memory impairment in a rat model of sepsis-associated encephalopathy via the NLRP3/Bax/Bcl pathway

Junzuo Gong

¹

,

Shu Luo

¹

,

Shiqiao Zhao

¹

,

Songlin Yin

¹

,

Xiaofeng Li

¹

,

Tianyi Mou

¹

Department of Emergency, Affiliated Hospital of North Sichuan Medical College, Sichuan Province, China

Folia Neuropathol 2019; 57 (4): 327-334

DOI: https://doi.org/10.5114/fn.2019.89856

Online publish date: 2019/11/18

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Introduction
The present investigation determined the protective effect of myricitrin against sepsis-associated encephalopathy in rats.

Material and methods
Sepsis was induced by cecal ligation and puncture (CLP); rats were treated with 30 or 100 mg/kg of myricitrin for 5 days prior to the induction of CLP. The effect of myricitrin was observed by determining the neurological function using the open field test and step-down inhibitory avoidance test. Cerebral oedema and the levels of inflammatory and oxidative stress mediators were determined in brain tissues. Moreover, the expression levels of Bcl-2, Bax, IκB-α, nuclear factor κB (NF-κB), caspase-3 and NLRP3 were estimated in brain tissues by Western blotting and the mRNA expression of NF-κB, caspase-3 and NLRP3 in brain tissues was estimated by real-time polymerase chain reaction. An immunofluorescence assay was performed to estimate inflammasome activity.

Results
The results suggest that treatment with myricitrin protects neuronal function in rats with CLP-induced sepsis. Decreases in inflammation and oxidative stress mediators were observed in the brain tissues of the myricitrin-treated group compared to the CLP group. Moreover, treatment with myricitrin ameliorated the altered Bcl-2, Bax, IκB-α, NF-κB, caspase-3 and NLRP3 protein and mRNA expression levels in the brain tissues of septic rats.

Conclusions
The data reveal that myricitrin ameliorated neuroinflammation and improved memory in rats with CLP-induced sepsis by regulating the NLRP3/Bax/Bcl signalling pathway.

keywords:

Myricitrin attenuates memory impairment in a rat model of sepsis-associated encephalopathy via the NLRP3/Bax/Bcl pathway

Junzuo Gong 1 , Shu Luo 1 , Shiqiao Zhao 1 , Songlin Yin 1 , Xiaofeng Li 1 , Tianyi Mou 1

myricitrin, neuroinflammation, sepsis, encephalopathy, oedema

Junzuo Gong

¹

,

Shu Luo

¹

,

Shiqiao Zhao

¹

,

Songlin Yin

¹

,

Xiaofeng Li

¹

,

Tianyi Mou

¹