eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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2/2020
vol. 45
 
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abstract:
Clinical immunology

Non-classical human leukocyte antigen class I in Tunisian children with autism

Najla Kharrat
1
,
Rania Abdelhedi
1
,
Imen Gtif
1
,
Imen Ayadi
1
,
Roberta Rizzo
1
,
Daria Bortolotti
1
,
Nouha Bouayed Abdelmoula
2
,
Farhat Ghribi
1, 3
,
Ahmed Rebai
1
,
Ines Zidi
4

  1. Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology of Sfax, Sfax University, Tunisia
  2. Research Unit: UR17ES36 Genomics of Signalopathies in the Service of Medicine, Sfax University, Medical University of Sfax, Tunisia
  3. Department of Child and Adolescent Psychiatry, Hédi Chaker University Hospital, Sfax, Tunisia
  4. Laboratory Microorganismes and Active Biomolecules, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia
Cent Eur J Immunol 2020; 45 (2): 176-183
DOI: https://doi.org/10.5114/ceji.2020.97906
Online publish date: 2020/07/27
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Autism spectrum disorders (ASD) are one of the most common childhood morbidities characterized by deficits in communication and social skills. Increasing evidence has suggested associations between immune genes located in the human leukocyte antigen (HLA) complex and etiology of autism.

In this study, we investigated whether the non-classical class I HLA-G, -E, and -F polymorphisms are associated with genetic predisposition to autism in Tunisia. We aimed to find a correlation between HLA-G genotypes and soluble HLA-G (sHLA-G) levels. We have analyzed the HLA-G, -E, and -F genotypes of 15 autistic children and their parents. DNA typing of HLA class I genes was performed using PCR-SSP and PCR-RFLP methods. Also, we evaluated the serum levels of HLA-G (1 and 5) by a validated ELISA technique in autistic probands and their parents.

No association was found between any polymorphism and autism in the study subjects. Additionally, we found no correlation between sHLA-G1 and sHLA-G5 and autism. Also, no significant difference in sHLA-G testing in parents and offspring was found. However, parents carrying [GG] genotype presented a higher sHLA-G levels than those carrying ([CC]+[GC]) genotypes (p = 0.037).

From this preliminary study, we conclude that the investigated polymorphisms of HLA-G, -E, and -F genes did not lead to autism susceptibility in Tunisian children. However, the CGTIGA haplotype was found to be associated with the disease.
keywords:

polymorphism, autism, HLA-G, HLA-E, HLA-F
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