Original article<br>Cellular mechanisms of white matter regeneration in an adult dysmyelinated rat model

Jacek M. Kwiecien

doi:10.5114/fn.2013.37703

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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3/2013
vol. 51

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abstract:

Original article
Cellular mechanisms of white matter regeneration in an adult dysmyelinated rat model

Jacek M. Kwiecien

Folia Neuropathol 2013; 51 (3): 189-202

DOI: https://doi.org/10.5114/fn.2013.37703

Online publish date: 2013/09/27

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Cellular mechanisms of regeneration after the white matter injury are difficult to study because of severe, inflammatory response to massively damaged myelin. Myelin-lacking CNS of the adult Long Evans Shaker (LES) rat supplies a model where neuroregeneration can be studied conveniently. The crush site in the dorsal spinal column in LES rats implanted with the normal rat choroid plexus was studied under the light and electron microscopy at 5 time points 3-56 days post-op. While the crush injury in normal rats resulted in severe inflammation active beyond 8 weeks, the same injury in LES rats resulted in a brief inflammation that resolved before day 7 post-op. In a clear fluid-filled crush cavity, ependymal cells from the implanted choroid plexus encased multiple regenerating axons, apparently guided them across the crush cavity and participated in establishing of a zone of neuroregeneration, morphologically similar to the white matter, at the interface of the crush cavity and the surrounding tissue of the spinal cord. Axons that were not encased by implanted cells failed to cross the crush cavity and persisted as markedly swollen end bulbs filled with organelles. At 8 weeks post-op, a large proportion of axons in the zone of neuroregeneration became myelinated by Schwann cells, likely originating from dorsal nerve roots or by oligodendrocytes that formed thin sheaths with a major dense line and likely originated from the implanted choroid plexus. The LES rat can serve as a convenient model to study mechanisms of neuroregeneration including axonal regeneration in the adult CNS injury.

keywords:

spinal cord injury, axonal regeneration, choroid plexus implant, inhibition of leukomyelitis, transdifferentiation of ependymal cells to oligodendrocytes, zone of neuroregeneration

Original articleCellular mechanisms of white matter regeneration in an adult dysmyelinated rat model

Jacek M. Kwiecien

spinal cord injury, axonal regeneration, choroid plexus implant, inhibition of leukomyelitis, transdifferentiation of ependymal cells to oligodendrocytes, zone of neuroregeneration

Original article
Cellular mechanisms of white matter regeneration in an adult dysmyelinated rat model