Original paper<br>Age-dependent neuroprotection of retinal ganglion cells by tempol-C8 acyl ester in a rat NMDA toxicity model

Michal Fiedorowicz; Robert Rejdak; Frank Schuettauf; Michal Wozniak; Pawel Grieb; Sebastian Thaler

doi:10.5114/fn.2014.45570

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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3/2014
vol. 52

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abstract:

Original paper
Age-dependent neuroprotection of retinal ganglion cells by tempol-C8 acyl ester in a rat NMDA toxicity model

Michal Fiedorowicz

,

Robert Rejdak

,

Frank Schuettauf

,

Michal Wozniak

,

Pawel Grieb

,

Sebastian Thaler

Folia Neuropathol 2014; 52 (3): 291-297

DOI: https://doi.org/10.5114/fn.2014.45570

Online publish date: 2014/09/26

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Background: The efficacy of tempol and its acyl derivative tempol-C8 as retinoprotective agents was compared in a rat model of NMDA-induced retinal ganglion cell (RGC) damage.

Material and methods: Tempol or tempol-C8 in different doses was administered intraperitoneally to 6 weeks old (pre-adolescent) and 9-10 weeks old (young adult) rats before and after an intravitreous NMDA injection. Retinal ganglion cell were retrogradely labeled with the fluorescent tracer hydroxystilbamidine and RGC counting was performed on retinal flatmounts.

Results: Intravitreal NMDA reduced RGC counts by about 90%, independently of age (p < 0.001). In pre-adolescent animals tempol-C8, but not tempol unmodified, showed a significant, dose-dependent RGC rescue effect, with peak activity at 5.8 µmol/kg (p < 0.001). In young adult animals, however, no neuroprotective effect was found for either tempol or tempol-C8.

Conclusions: In contrast to tempol itself, tempol-C8 acyl ester was neuroprotective in pre-adolescent rats in the NMDA- induced RGC damage model. Therefore, neuroprotection by tempol acyl esters seems to be superior to that of tempol under certain conditions.

keywords:

Original paperAge-dependent neuroprotection of retinal ganglion cells by tempol-C8 acyl ester in a rat NMDA toxicity model

Michal Fiedorowicz , Robert Rejdak , Frank Schuettauf , Michal Wozniak , Pawel Grieb , Sebastian Thaler

tempol, tempol acyl ester, NMDA, ganglion cell, neuroprotection

Original paper
Age-dependent neuroprotection of retinal ganglion cells by tempol-C8 acyl ester in a rat NMDA toxicity model

Michal Fiedorowicz

,

Robert Rejdak

,

Frank Schuettauf

,

Michal Wozniak

,

Pawel Grieb

,

Sebastian Thaler