eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
1/2021
vol. 46
 
Share:
Share:
abstract:
Experimental immunology

PDCD4-mediated downregulation of Listeria monocytogenes burden in macrophages

Xingju Zhang
1, 2
,
Jiale Zhang
3
,
Fei Li
4
,
Yachen Luo
5
,
Shan Jiang
2

  1. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China
  2. Institute for Advanced Study, Shenzhen University, Shenzhen, China
  3. School of Pharmaceutical Sciences and Innovative Drug Research Center, Chongqing University, Chongqing, China
  4. Biomedical Analysis Center, Army Medical University, Chongqing, China
  5. Frederick S. Pardee School of Global Studies, Boston University, Boston, USA
Cent Eur J Immunol 2021; 46 (1): 38-46
Online publish date: 2021/04/18
View full text Get citation
 
PlumX metrics:
Introduction
Macrophages are effector cells of the innate immune system and defend against invading pathogens. Previous reports have shown that infection with Listeria monocytogenes upregulates miR-21a expression in macrophages. Aim of the study: We aimed to verify whether programmed cell death 4 (PDCD4) is involved in the high bacterial burden observed in macrophages during late-stage L. monocytogenes infections.

Material and methods
We examined the expression of miR-21a and its known target PDCD4 in macrophages after L. monocytogenes infection. The macrophages’ uptake ability of L. monocytogenes was measured using FluoSpheres Carboxylate-modified microspheres. We depleted PDCD4 by transfecting macrophages with siPDCD4.

Results
In macrophages, PDCD4 protein was downregulated 5 h, but not 2 h, after L. monocytogenes infection. Our results validated the hypothesis that PDCD4-depleted macrophages present a higher L. monocytogenes burden. Moreover, we found that the activation of c-Jun and STAT3 accompanied PDCD4 downregulation.

Conclusions
Our results showed that PDCD4 mediated the suppression of L. monocytogenes infection in macrophages via c-Jun/STAT3 signalling activation.

keywords:

Listeria monocytogenes, macrophage, PDCD4

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.