PDCD4-mediated downregulation of Listeria
monocytogenes burden in macrophages

Xingju Zhang; Jiale Zhang; Fei Li; Yachen Luo; Shan Jiang

doi:10.5114/ceji.2021.105244

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

Experimental immunology

PDCD4-mediated downregulation of Listeria monocytogenes burden in macrophages

Xingju Zhang

^{1, 2}

,

Jiale Zhang

³

,

Fei Li

⁴

,

Yachen Luo

⁵

,

Shan Jiang

²

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China
Institute for Advanced Study, Shenzhen University, Shenzhen, China
School of Pharmaceutical Sciences and Innovative Drug Research Center, Chongqing University, Chongqing, China
Biomedical Analysis Center, Army Medical University, Chongqing, China
Frederick S. Pardee School of Global Studies, Boston University, Boston, USA

Cent Eur J Immunol 2021; 46 (1): 38-46

DOI: https://doi.org/10.5114/ceji.2021.105244

Online publish date: 2021/04/18

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Introduction
Macrophages are effector cells of the innate immune system and defend against invading pathogens. Previous reports have shown that infection with Listeria monocytogenes upregulates miR-21a expression in macrophages. Aim of the study: We aimed to verify whether programmed cell death 4 (PDCD4) is involved in the high bacterial burden observed in macrophages during late-stage L. monocytogenes infections.

Material and methods
We examined the expression of miR-21a and its known target PDCD4 in macrophages after L. monocytogenes infection. The macrophages’ uptake ability of L. monocytogenes was measured using FluoSpheres Carboxylate-modified microspheres. We depleted PDCD4 by transfecting macrophages with siPDCD4.

Results
In macrophages, PDCD4 protein was downregulated 5 h, but not 2 h, after L. monocytogenes infection. Our results validated the hypothesis that PDCD4-depleted macrophages present a higher L. monocytogenes burden. Moreover, we found that the activation of c-Jun and STAT3 accompanied PDCD4 downregulation.

Conclusions
Our results showed that PDCD4 mediated the suppression of L. monocytogenes infection in macrophages via c-Jun/STAT3 signalling activation.

keywords:

PDCD4-mediated downregulation of Listeria monocytogenes burden in macrophages

Xingju Zhang 1, 2 , Jiale Zhang 3 , Fei Li 4 , Yachen Luo 5 , Shan Jiang 2

Listeria monocytogenes, macrophage, PDCD4

Xingju Zhang

^{1, 2}

,

Jiale Zhang

³

,

Fei Li

⁴

,

Yachen Luo

⁵

,

Shan Jiang

²