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Anaesthesiology Intensive Therapy
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5/2022
vol. 54
 
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abstract:
Original paper

Parenteral succinate reduces levels of reactive oxygen species without changing serum caspase-3 levels in septic rats

Sebastián Chapela
1, 2
,
Giovanna Muscogiuri
3, 4
,
Luigi Barrea
4, 5
,
Evelyn Frias-Toral
6
,
Hilda Burgos
1
,
María Cecilia Ricart
7
,
Alexis Muryan
8
,
Amalia Schiel
8
,
Manuel Alonso
9
,
Carlos Alberto Stella
1

  1. Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
  2. Hospital Británico de Buenos Aires, Buenos Aires, Argentina
  3. Dipartimento di Medicina Clinica e Chirurgia, Unit of Endocrinology, Federico II University Medical School of Naples, Naples, Italy
  4. Department of Clinical Medicine and Surgery, Centro Italiano per la cura e il Benessere del paziente con Obesità (C.I.B.O), University Medical School of Naples, Naples, Italy
  5. Dipartimento di Scienze Umanistiche, Università Telematica Pegaso, Naples, Italy
  6. Universidad Espíritu Santo, Samborondón, Ecuador
  7. Cátedra de Química Biológica, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, Argentina
  8. Laboratorio Central, Hospital Británico de Buenos Aires, Buenos Aires, Argentina
  9. Departamento de Ciencias Biológicas, Ciclo Básico Común, Universidad de Buenos Aires, Buenos Aires, Argentina
Anaesthesiol Intensive Ther 2022; 54, 5: 357–364
DOI: https://doi.org/10.5114/ait.2022.122549
Online publish date: 2022/12/30
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Introduction
Sepsis is a syndrome of physiological, pathological, and biochemical disorders with several processes co-occurring; reactive oxygen species (ROS) production and apoptosis are 2 of them. Succinate is a Krebs cycle intermediate that is oxydized in complex II of the mitochondria. This study aims to investigate the influence of succinate infusion on these processes.

Material and methods
Sepsis was induced with caecal ligation and puncture in 200 gr Sprague Dawley rats. Four groups were formed with 10 animals (1 – control, 2 – succinate, 3 – sepsis, and 4 – sepsis + succinate). 5 mmol kg–1 of intraperitoneal succinate were administered twice in groups 2 and 4. ROS and caspase-3 levels were measured.

Results
Overall, ROS levels (P = 0.017), but not caspase-3 levels (P = 0.89) differed significantly between the groups. The succinate administration reduced serum ROS levels (group 4 vs. 3) in a statistically significant way [0.0623 units (95% CI: 0.0547-0.0699) vs. 0.0835 (0.06-0.106), P = 0.017)], but it did not reduce serum caspase-3 levels (P = 0.39). There was no correlation between serum ROS levels and serum caspase-3 levels.

Conclusions
In this model, ROS levels were reduced with succinate infusion, but caspase-3 levels were not. In addition, ROS levels and apoptosis levels are not correlated, which suggests that those processes occur at different times.

keywords:

sepsis, succinate, apoptosis, reactive oxygen species, rats
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