eISSN: 1509-572x
ISSN: 1641-4640
Folia Neuropathologica
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4/2016
vol. 54
 
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abstract:
Original paper

Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cells

Chen Chen
1
,
Yanning Qian
2

  1. Department of Anesthesiology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210000, China
  2. Department of Anesthesiology, The First People’s Hospital of Changzhou, Changzhou, Jiangsu, 213000, China
Folia Neuropathol 2016; 54 (4): 382-391
Online publish date: 2016/12/27
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Unmethylated CpG DNA, as a stimulatory molecule, has potent pro-inflammatory effects in the central nervous system (CNS). Dexmedetomidine (DEX) has been confirmed to exert anti-inflammatory effects in CNS. Our study was aimed to explore the effects of DEX on tumor necrosis factor-α (TNF-α) expression in unmethylated CpG DNA-challenged microglia. In vivo, after 3 d intracisternal injection of ODN1668, we evaluated the severity of meningitis with or without DEX via pathobiology method and detected the expression of TNF-α from molecular and protein levels. In vitro, we explored whether the ODN1668 could activate microglia to express TNF-α and the inhibition mechanism of DEX. Our results demonstrated that DEX could alleviate the severity of ODN1668-induced meningitis. And while BV2 microglia was stimulated by ODN1668 for different time, TNF-α was increased in mRNA and protein levels but the effect was attenuated by DEX via decreasing phosphorylated AKT and ERK
keywords:

DEX, unmethylated CpG DNA, microglia

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