Pseudopterosin A ameliorates ischaemia-induced brain injury by acting on Akt signalling pathway

Xinkai Niu; Chaochun Yu; Guanfu Jiang; Jun Wu; Xiaohui Tan; Weijia Zhang; Lijun Hou

doi:10.5114/fn.2018.76614

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures

Editorial System

Submit your Manuscript

Editorial Policies

Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

2/2018
vol. 56

Send email

Copy url:

abstract:

Original paper

Pseudopterosin A ameliorates ischaemia-induced brain injury by acting on Akt signalling pathway

Xinkai Niu

,

Chaochun Yu

,

Guanfu Jiang

,

Jun Wu

,

Xiaohui Tan

,

Weijia Zhang

,

Lijun Hou

Folia Neuropathol 2018; 56 (2): 104-111

DOI: https://doi.org/10.5114/fn.2018.76614

Online publish date: 2018/06/28

View full text Get citation

PlumX metrics:

Introduction
Brain injury caused by ischaemic stroke is a major cause of disability and death throughout the world. The present study evaluates the neuroprotective effect of pseudopterosin A (PtA) against ischaemia-induced brain injury.

Material and methods
Ischaemia was induced by pMCAO model, and rats were separated in to three groups. A control group; an ischemic group receiving saline solution; and a PtA group receiving PtA (5 mg/kg, i.p.) for the period of three days, i.e. two days before surgery and after the surgery. Effect of PtA was assessed by estimating the infract volume and neurological deficits. Level of oxidative stress, inflammatory mediators, and markers of apoptosis were estimated in the brain tissues. Western blot assay and immunohistochemistry was done for the assessment of expressions of protein.

Result
Data from the study suggest that treatment with PtA significantly decreases the infracted volume and neurological deficit score compared to the ischaemic group. Treatments with PtA attenuate the activity of antioxidant enzyme, and the level of inflammatory mediators and markers of apoptosis in the brain tissues of ischaemia-induced brain injury. Phosphorylation of FKHR, Bad, and Akt were significantly reduced in the PtA-treated group compared to the ischaemic group.

Conclusions
The present study shows that pseudopterosin A attenuates neuronal injury in the pMCAO model by acting on the Akt signalling pathway.

keywords:

Pseudopterosin A ameliorates ischaemia-induced brain injury by acting on Akt signalling pathway

Xinkai Niu , Chaochun Yu , Guanfu Jiang , Jun Wu , Xiaohui Tan , Weijia Zhang , Lijun Hou

pseudopterosin A, brain injury, Akt signalling, apoptosis

Xinkai Niu

,

Chaochun Yu

,

Guanfu Jiang

,

Jun Wu

,

Xiaohui Tan

,

Weijia Zhang

,

Lijun Hou