Reference values of lymphocyte subsets from healthy Polish adults

Aleksander Roszczyk; Michał Zych; Dariusz Sołdacki; Radoslaw Zagozdzon; Monika J. Kniotek

doi:10.5114/ceji.2024.136371

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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vol. 49

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abstract:

Clinical immunology

Reference values of lymphocyte subsets from healthy Polish adults

Aleksander Roszczyk

¹

,

Michał Zych

¹

,

Dariusz Sołdacki

²

,

Radoslaw Zagozdzon

^{1, 3}

,

Monika J. Kniotek

¹

Department of Clinical Immunology, Medical University of Warsaw, Poland
Longevity Center, Warsaw, Poland
Department of Bioinformatics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

Cent Eur J Immunol 2024; 49 (1): 26-36

DOI: https://doi.org/10.5114/ceji.2024.136371

Online publish date: 2024/03/22

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The flow cytometry method could support physicians’ decisions in the diagnosis and treatment monitoring of immunodeficient patients. Most clinical recommendations are focused on the search for alterations in T- and B-lymphocyte subsets, less commonly natural killer (NK) cells and granulocytes. While reference values for clinically meaningful lymphocyte subsets have been published ubiquitously among numerous countries, we have not found significant data for a population of adult Polish habitats; thus we determined reference values for T, B, and NK subsets according to sex and age. The female group showed a higher percentage of lymphocytes (CD45⁺⁺), T helper lymphocytes with a higher absolute count, as well as CD4/CD8 ratio, marginal zone-like B cells, class-switched B cells, and CD21^low B cells than the male group. The male group was found to have elevated percentages of naïve B lymphocytes, transitional B cells, and plasmablasts. A weak positive correlation with age was found among double positive T lymphocytes, natural killer T cells (NKT) lymphocytes, and CD21^low B cells. A negative correlation with age for double negative T lymphocytes, marginal zone-like B cells, and plasmablasts was noted. The results indicated the importance of creating distinct reference ranges regarding sex and age concerning immunophenotype.

keywords:

B lymphocytes subsets, class-switching B lymphocytes, CVID – common variable immunodeficiency, flow cytometry, healthy adults, immunophenotype, lymphocyte subsets, peripheral blood, NK – natural killer, NKT – natural killer T cells, primary immunodeficiency – PID, reference values

Reference values of lymphocyte subsets from healthy Polish adults

Aleksander Roszczyk 1 , Michał Zych 1 , Dariusz Sołdacki 2 , Radoslaw Zagozdzon 1, 3 , Monika J. Kniotek 1

B lymphocytes subsets, class-switching B lymphocytes, CVID – common variable immunodeficiency, flow cytometry, healthy adults, immunophenotype, lymphocyte subsets, peripheral blood, NK – natural killer, NKT – natural killer T cells, primary immunodeficiency – PID, reference values

Aleksander Roszczyk

¹

,

Michał Zych

¹

,

Dariusz Sołdacki

²

,

Radoslaw Zagozdzon

^{1, 3}

,

Monika J. Kniotek

¹