The dynamic expression of aquaporins 1 and 4 in rats with hydrocephalus induced by subarachnoid haemorrhage

Chun-Yan Long; Gui-Qin Huang; Qiong Du; Li-Qing Zhou; Jing-Hua Zhou

doi:10.5114/fn.2019.86296

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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2/2019
vol. 57

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abstract:

The dynamic expression of aquaporins 1 and 4 in rats with hydrocephalus induced by subarachnoid haemorrhage

Chun-Yan Long

^{1, 2}

,

Gui-Qin Huang

³

,

Qiong Du

¹

,

Li-Qing Zhou

⁴

,

Jing-Hua Zhou

^{1, 2}

Department of Neurology, The First College of Clinic Medical Sciences, China Three Gorges University, Yichang 443003, Hubei Province, China
Institute of Neurology, China Three Gorges University, Yichang 443003, Hubei Province, China
Experimental Animal Center, China Three Gorges University, Yichang 443002, Hubei Province, China
Zigui Maternal and Child Health Care Hospital, Yichang 443003, Hubei Province, China

Folia Neuropathol 2019; 57 (2): -182-195

DOI: https://doi.org/10.5114/fn.2019.86296

Online publish date: 2019/06/28

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Introduction
Hydrocephalus is a common complication of subarachnoid haemorrhage (SAH). As transmembrane water channels, aquaporins 1 and 4 (AQP1 and AQP4) are involved in the pathogenesis of hydrocephalus. We aimed to assess the association between the expressions of AQP1 and AQP4 and the severity and duration of hydrocephalus after SAH.

Material and methods
A double haemorrhage model by injection of autologous blood into the cisterna magna was used to induce SAH in rats. Sham rats received the same procedures, but with the injection of normal saline. The SAH group was divided into the SAH with hydrocephalus group and SAH without hydrocephalus group after identifying hydrocephalus histologically. AQP1 and AQP4 in ventricle regions were detected by immunofluorescence, quantitative polymerase chain reaction (qPCR) and western blot.

Results
Hydrocephalus was the most severe at day 3 after SAH. AQP1 and AQP4 mRNA and protein levels increased at day 1 and peaked at day 3. The SAH with hydrocephalus group had a higher expression of AQP1 and AQP4 than the SAH without hydrocephalus group. Higher AQP1 levels were found at the apical and basolateral membrane of the choroid plexus epithelium, while higher AQP4 levels were found in the ependymal cells. A positive correlation between the relative lateral ventricle area and the ratio of AQP1/AQP4 proteins was identified.

Conclusions
AQP1 and AQP4 are remarkably correlated with the severity of hydrocephalus induced by SAH. AQP1 and AQP4 are potential drug targets for developing therapeutic strategies against hydrocephalus.

keywords:

The dynamic expression of aquaporins 1 and 4 in rats with hydrocephalus induced by subarachnoid haemorrhage

Chun-Yan Long 1, 2 , Gui-Qin Huang 3 , Qiong Du 1 , Li-Qing Zhou 4 , Jing-Hua Zhou 1, 2

subarachnoid haemorrhage, hydrocephalus, aquaporin-1, aquaporin-4

Chun-Yan Long

^{1, 2}

,

Gui-Qin Huang

³

,

Qiong Du

¹

,

Li-Qing Zhou

⁴

,

Jing-Hua Zhou

^{1, 2}