The possibility of the clinical use of keratinocytes and epidermal stem cells cultures
in the treatment of chronic leg ulcers – preliminary report

Beata Imko-Walczuk; Aleksandra Okuniewska; Michał Pikuła; Danuta Nowacka-Pikuła; Maria Czubek; Janusz Jaśkiewicz; Piotr Trzonkowski

eISSN: 2084-9893
ISSN: 0033-2526

Dermatology Review/Przegląd Dermatologiczny

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3/2012
vol. 99

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abstract:

Original paper

The possibility of the clinical use of keratinocytes and epidermal stem cells cultures in the treatment of chronic leg ulcers – preliminary report

Beata Imko-Walczuk

,

Aleksandra Okuniewska

,

Michał Pikuła

,

Danuta Nowacka-Pikuła

,

Maria Czubek

,

Janusz Jaśkiewicz

,

Piotr Trzonkowski

Przegl Dermatol 2012, 99, 230–234

Online publish date: 2012/06/25

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AMA

Imko-Walczuk B, Okuniewska A, Pikuła M, et al. The possibility of the clinical use of keratinocytes and epidermal stem cells cultures
in the treatment of chronic leg ulcers – preliminary report. Dermatology Review/Przegląd Dermatologiczny. 2012;99(3):230-234.

APA

Imko-Walczuk, B., Okuniewska, A., Pikuła, M., Nowacka-Pikuła, D., Czubek, M.,  & Jaśkiewicz, J. et al. (2012). The possibility of the clinical use of keratinocytes and epidermal stem cells cultures
in the treatment of chronic leg ulcers – preliminary report. Dermatology Review/Przegląd Dermatologiczny, 99(3), 230-234.

Chicago

Imko-Walczuk, Beata, Aleksandra Okuniewska, Michał Pikuła, Danuta Nowacka-Pikuła, Maria Czubek, Janusz Jaśkiewicz,  and Piotr Trzonkowski. 2012. "The possibility of the clinical use of keratinocytes and epidermal stem cells cultures
in the treatment of chronic leg ulcers – preliminary report". Dermatology Review/Przegląd Dermatologiczny 99 (3): 230-234.

Harvard

Imko-Walczuk, B., Okuniewska, A., Pikuła, M., Nowacka-Pikuła, D., Czubek, M., Jaśkiewicz, J.,  and Trzonkowski, P. (2012). The possibility of the clinical use of keratinocytes and epidermal stem cells cultures
in the treatment of chronic leg ulcers – preliminary report. Dermatology Review/Przegląd Dermatologiczny, 99(3), pp.230-234.

MLA

Imko-Walczuk, Beata et al. "The possibility of the clinical use of keratinocytes and epidermal stem cells cultures
in the treatment of chronic leg ulcers – preliminary report." Dermatology Review/Przegląd Dermatologiczny, vol. 99, no. 3, 2012, pp. 230-234.

Vancouver

Imko-Walczuk B, Okuniewska A, Pikuła M, Nowacka-Pikuła D, Czubek M, Jaśkiewicz J et al. The possibility of the clinical use of keratinocytes and epidermal stem cells cultures
in the treatment of chronic leg ulcers – preliminary report. Dermatology Review/Przegląd Dermatologiczny. 2012;99(3):230-234.

Introduction. Treatment of chronic leg ulcers is still a serious problem in modern medicine. Therapeutic failures are observed both in patients treated conservatively and in those in whom surgical treatment was undertaken. Therefore there is a need of new methods of therapy.

Objective. To present clinical use of cultured keratinocytes and epidermal stem cells in two patients with chronic leg ulcers. The authors also attempted to determine the clinical criteria for inclusion and exclusion of patients for this type of therapy.

Material and methods. A cell suspension from full-thickness skin was obtained from both patients and placed in the bottom of the ulcers. The wounds were protected with sterile dressings soaked in saline.

Results. In both patients partial re-epithelialisation of the ulcer and significant clinical improvement around the wound were observed.

Conclusions. In spite of clinical improvement observed in both cases, the authors draw attention to the need of improvement of cell culture technique and development of clinical criteria for this therapeutic procedure.

keywords:

The possibility of the clinical use of keratinocytes and epidermal stem cells cultures in the treatment of chronic leg ulcers – preliminary report

Beata Imko-Walczuk , Aleksandra Okuniewska , Michał Pikuła , Danuta Nowacka-Pikuła , Maria Czubek , Janusz Jaśkiewicz , Piotr Trzonkowski

leg ulcers, therapy of chronic wounds, keratinocyte culture

Beata Imko-Walczuk

,

Aleksandra Okuniewska

,

Michał Pikuła

,

Danuta Nowacka-Pikuła

,

Maria Czubek

,

Janusz Jaśkiewicz

,

Piotr Trzonkowski