The protective effect of Wnt3a on inflammatory response in oxygen-glucose deprivation/reoxygenation (OGD/R) astrocyte model

Su-Qin Chen; Ang Li; Min-Yan Feng; Wen-Qing Zhong; Liu Yang; Xiao-Yu Chen; Xing-Er Wu

doi:10.5114/fn.2023.130264

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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3/2023
vol. 61

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abstract:

Original paper

The protective effect of Wnt3a on inflammatory response in oxygen-glucose deprivation/reoxygenation (OGD/R) astrocyte model

Su-Qin Chen

^{1, 2}

,

Ang Li

³

,

Min-Yan Feng

⁴

,

Wen-Qing Zhong

⁵

,

Liu Yang

⁶

,

Xiao-Yu Chen

¹

,

Xing-Er Wu

⁷

Department of Neurology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China
Department of Neurology, Huizhou Central People’s Hospital, Huizhou City, Guangdong, China
Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China
Department of Clinical Psychology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China
Department of Special Medical Center, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China
Department of Stomatology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China
Department of Laboratory Medicine, Zhongshan City People’s Hospital, Zhongshan, Guangdong, China

Folia Neuropathol 2023; 61 (3): 242-248

DOI: https://doi.org/10.5114/fn.2023.130264

Online publish date: 2023/08/04

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Involving in the immune response after cerebral infarction, astrocytes could secrete large amounts of pro- and anti-inflammatory factors. The aim of this study is to investigate the effect of Wnt3a intervention on the inflammatory response of oxygen-glucose deprivation (OGD) followed by reoxygenation (OGD/R) astrocyte model, and to provide a new target for immunoprotective treatment of cerebral infarction. We constructed the OGD/R rat astrocyte model, the astrocytes were treated by different concentrations of glucose (25, 50, 100 mM) intervened with/without Wnt3a (25 µg/ml). Microscope was used to observe the cell survival in rat astrocytes. The relative expression of inflammatory factors (TNF-a, IL-6, HIF-a) in rat astrocytes was detected by qRT-PCR. The expression of inflammatory factors such as TNF-a, IL-6 and HIF-a in rat astrocytes was increased after OGD/R treatment. The Wnt3a intervention promoted cell survival and decreased the expression of inflammatory factors in rat astrocytes induced by OGD/R. There is a neuroprotective effect that Wnt3a intervention could reduce inflammatory response in the OGD/R rat astrocyte model.

keywords:

The protective effect of Wnt3a on inflammatory response in oxygen-glucose deprivation/reoxygenation (OGD/R) astrocyte model

Su-Qin Chen 1, 2 , Ang Li 3 , Min-Yan Feng 4 , Wen-Qing Zhong 5 , Liu Yang 6 , Xiao-Yu Chen 1 , Xing-Er Wu 7

Wnt3a, astrocyte, oxygen-glucose deprivation (OGD/R), inflammatory factors

Su-Qin Chen

^{1, 2}

,

Ang Li

³

,

Min-Yan Feng

⁴

,

Wen-Qing Zhong

⁵

,

Liu Yang

⁶

,

Xiao-Yu Chen

¹

,

Xing-Er Wu

⁷