The role of myeloid differentiation factor 88 on mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis

Yu Gong; Lin Zou; Dunjin Chen; Wei Chao

doi:10.5114/ceji.2016.60989

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2016
vol. 41

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abstract:

Experimental immunology

The role of myeloid differentiation factor 88 on mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis

Yu Gong

,

Lin Zou

,

Dunjin Chen

,

Wei Chao

(Cent Eur J Immunol 2016; 41 (2): 153-158)

DOI: https://doi.org/10.5114/ceji.2016.60989

Online publish date: 2016/07/15

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Objective: To investigate the role of myeloid differentiation factor 88 (MyD88) on mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis.

Material and methods: Polymicrobial peritonitis, a clinically relevant mouse model of sepsis, was generated by cecum ligation and puncture (CLP) in both male C57BL/6J wild-type (WT) and MyD88 knockout (MyD88^–/–) mice. Twenty-four hours after surgeries, peritoneal leukocytes were collected and four parameters of mitochondrial function, including total intracellular and mitochondrial ROS burst, mitochondrial membrane depolarization and ATP depletion, were measured by flow cytometry or ATP assay, and then compared.

Results: Polymicrobial sepsis led to a marked mitochondrial dysfunction of peritoneal leukocytes with total intracellular and mitochondrial ROS overproduction, decreased mitochondrial membrane potential and reduced intracellular ATP production. In comparison, there was no significant difference in the extent of mitochondrial dysfunction of peritoneal leukocytes between WT and MyD88^–/– septic mice.

Conclusions: MyD88 may be not sufficient to regulate mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis.

keywords:

The role of myeloid differentiation factor 88 on mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis

Yu Gong , Lin Zou , Dunjin Chen , Wei Chao

polymicrobial sepsis, peritoneal leukocyte, mitochondrial dysfunction, MyD88

Yu Gong

,

Lin Zou

,

Dunjin Chen

,

Wei Chao