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ISSN: 2392-1099
Clinical and Experimental Hepatology
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3/2020
vol. 6
 
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abstract:
Original paper

The superior role of coagulation factor FX over FVII in adenoviral-mediated innate immune induction of the hepatocyte: an in vitro experiment

Saeed Firoozi Ghahestani
1
,
Alireza Shiri
1
,
Afagh Moattari
1
,
Jamal Sarvari
1, 2
,
Ali Moahammad Tamaddon
3, 4
,
Seyed Younes Hosseini
1

  1. Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran
  2. Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  3. Pharmaceutics Department, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
  4. Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz, Iran
Clin Exp HEPATOL 2020; 6, 3: 199–206
DOI: https://doi.org/10.5114/ceh.2020.99512
Online publish date: 2020/09/30
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Introduction
To better understanding the contribution of coagulation factors to the extent of adenovirus-mediated innate toxicity on the hepatocyte.

Material and methods
Adenovirus-36 (AD) and adenovector type 5-GFP (Ad5-GFP) were propagated and titered; then, they were loaded with coagulation factors VII or X. The complex of adenovirus with coagulation factor VII and X were for size and charge parameters. After adding AD-VII and AD-X complexes, the expression levels of innate inflammatory genes including protein kinase R (PKR), interleukin (IL)-1, IL-8 and IL-18 were measured by Real-time PCR on a hepatocellular carcinoma cell line, HepG2.

Results
: The loading of coagulation factors VII and X on Ad5-GFP enhanced the transduction rate up to 50% and 60% (p < 0.05), respectively, compared to the adenovector alone (30%) (p < 0.05). The formation of the coagulation factor-virus complex leads to multimodal size distribution with an increase in average hydrodynamic size and absolute zeta potential. The qPCR results showed that PKR expression increased significantly after treatment with all adenoviruses. These findings also showed that AD had a significant (p = 0.0152) inflammatory impact on Hep-G2. However, AD which was loaded with FX (AD-X) exhibited the most inflammatory effect (p = 0.0164). Significantly, the expression of IL-1 (p = 0.0041), IL-8 (p = 0.0107) and IL-18 (p = 0.0193) were also enhanced following FX loading. On the other hand, the AD-VII complex showed the least effect of innate immune induction when compared to the negative control (p < 0.05).

Conclusions
The loading of coagulation factors, particularly FX, could enhance the transduction efficiency of Ad5-GFP. Furthermore, adenovirus loaded with FX exhibited more innate toxicity on the hepatocytes, while it was not the case for FVII.

keywords:

innate immunity, adenovirus, gene therapy, hepatocyte, coagulation factor
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